大连美仑生物技术有限公司

Apoptosis Bcl-2 Bcl-6 Caspase Ferroptosis Mdm2 p53 RIP kinase Survivin TNF-alpha Cuproptosis IAP Metabolic Enzyme/Protease PDHK ROS Kinase Phosphatase MDM-2/p53 Kinesin PKC HSP Autophagy TNF Receptor FKBP Bcl-2 Family Pyroptosis DAPK PKD EBV Glutathione Peroxidase Transferrin Receptor c-Myc Necroptosis ASK1 Thymidylate Synthase

Autophagy

Autophagy LRRK2 Estrogen/progestogen PINK1/Parkin Dynamin Anti-infection Oxidative Phosphorylation Akt AMPK JAK Toll-like Receptor (TLR) ULK p62 Atg8/LC3 Atg7 Atg4 AUTACs

Epigenetics

DNA Methyltransferase DNMT Epigenetic Reader Domain Histone Acetyltransferase Histone demethylases Histone Methyltransferase PKC AMPK HDAC JAK MicroRNA Protein Arginine Deiminase Phosphodiesterase (PDE)

GPCR/G Protein

Adenosine Receptor Angiotensin Receptor Cannabinoid Receptor CaSR E1 Activating CRFR E2 conjugating E3 Ligase Endothelin Receptor GHSR Glucagon Receptor GLP-1R GPCR19(TGR5) GPR GPR119 GPR40(FFAR1) Imidazoline Receptor Leukotriene Receptor LPA Receptor OX Receptor Protease-activated Receptor Rac Prostaglandin Receptor Oxytocin Receptor Ras Rho S1P Receptor Sigma Receptor 5-HT Receptor Neurokinin Receptor mAChR GCGR LPL Receptor Dopamine Receptor Guanylate Cyclase Histamine Receptor CXCR CCR GPR35 P2Y Receptor Opioid Receptor GPR68 mGluR Formyl Peptide Receptor (FPR) Platelet-activating Factor Rec GnRH Receptor Protease Activated Receptor (P Vasopressin Receptor Chemerin Receptor Melatonin Receptor MCHR1 (GPR24) Orexin Receptor (OX Receptor) RGS Protein CGRP Receptor Free Fatty Acid Receptor

Immunology/Inflammation

AhR CCR COX Complement 5(C5) CXCR Histamine Receptor IL Receptor Immunology IL-5 NOD1 IL-12 PD-1 IL-17 PDL-1 reactive oxygen species (ROS) NLRP3 TLR STING Toll-like Receptor (TLR) Bcr-Abl Salt-inducible Kinase (SIK) Glucocorticoid Receptor IRAK SphK Interleukin Related PGE synthase Macrophage migration inhibitor Complement System MyD88 FLAP Thrombopoietin Receptor Arginase NO Synthase AP-1 Nuclear Factor of activated T IFNAR CD276/B7-H3 CD3 CD38 CD28 CD22 CD20 CD19 Aryl Hydrocarbon Receptor PD-1/PD-L1 NOD-like Receptor (NLR) Mesothelin Mucin NTPDase PSMA SOD DOCK CD74 CD6 CD73 CTLA-4 CX3CR1 Cyclic GMP-AMP Synthase FAP Galectin Cyclophilin P-selectin CD2 CD47 Tim3 TROP2 Glycoprotein VI Kallikrein LAG-3 VAP-1 BCL6

Integrin

JAK-STAT/IL6

JAK STAT IL-6 Pim EGFR

MAPK

ErbB2 ERK JNK MEK p38 MAPK Mixed Lineage Kinase Raf

Membrane Transporter/Ion Chann

Amino acid transporter antagon ATPase Calcium Channel CFTR Chloride channel CRM1 GABA Receptor GluR iGluR NMDAR P-gp Potassium Channel Proton Pump SGLT Sodium Channel TRPV P-glycoprotein Na+/K+ ATPase Piezo Channel Fat Mass and Obesity-associate TRP Channel BCRP nAChR CRAC Channel Apical Sodium-Dependent Bile A P2X Receptor Monocarboxylate Transporter Na+/HCO3- Cotransporter GlyT OAT NKCC Monoamine Transporter HCN Channel EAAT Ferroportin GLUT Na+/H+ Exchanger (NHE) Na+/Ca2+ Exchanger FATP ATP Synthase ASCT VDAC Urea Transporter URAT1 Aquaporin Calmodulin

Metabolic Enzyme/Protease

5-alpha Reductase Aminopeptidase ATP-citrate lyase in Carbonic Anhydrase CETP DGAT DPP-4 Factor Xa FOX FXR Glucokinase(GK) GSNOR HCV Protease HIF HMG-CoA Reductase HSP IDO Lactate Dehydrogenase Integrase MMP NAMPT P450 PAI-1 PDE PGC-1α Phospholipase Procollagen C Protein Proteases PKM Proteasome RAR/RXR Retinoid Receptor Serine Protease Thrombin Transferase Xanthine Oxidase p38-MAPK Stearoyl-CoA Desaturase (SCD) Mitochondrial Metabolism (MPC) Tyrosinase Endogenous Metabolite Phosphatase Pyruvate Kinase PDI Phosphodiesterase (PDE) HIF/HIF Prolyl-Hydroxylase Hexokinase Ser/Thr Protease Carboxylesterase Cathepsin Cytochrome P450 Drug Metabolite E1/E2/E3 Enzyme Angiotensin-converting Enzyme HMG-CoA Reductase (HMGCR) Aldose Reductase Oxidative Phosphorylation NADPH Oxidase Lipoxygenase DAGL MAGL MALT1 Adenosine Kinase COMT Glutathione S-transferase Adenosine Deaminase Acyltransferase Enolase Aldehyde Dehydrogenase (ALDH) Farnesyl Transferase Glucosidase PDHK Fatty Acid Synthase (FASN) Dipeptidyl Peptidase Methylenetetrahydrofolate Dehy Dihydroorotate Dehydrogenase Renin FAAH SGK Dopamine β-hydroxylase HIV Integrase Adiponectin Receptor ROR LXR Mineralocorticoid Receptor REV-ERB Amine N-methyltransferase

Neuronal Signaling

5-HT Receptor AChR BACE Beta Amyloid Beta-secretase CaMK CGRP Receptor Dopamine Receptor FAAH Gamma-secretase GlyT iGluR MAO mAChR Opioid Receptor Serotonin Transporter Legumain γ-secretase Notch Monoamine Oxidase Trk Receptor Cholinesterase (ChE) Imidazoline Receptor Amyloid-β Dopamine Transporter Sigma Receptor Glucosylceramide Synthase (GCS

NF-κB

NF-κB Nrf2 IκB/IKK Reactive Oxygen Species Keap1-Nrf2 IKK RANKL/RANK

Others

Androgen Receptor Aromatase CD20 CD52 c-Myc CPT1 c-RET CTLA-4 Decarboxylase Dehydrogenase FPR GLUT Glutaminase HIV ProteaseAnti-infection HSP90 Hydroxylase IAP IgE IRE1 LDL Lipase Lipoxygenase Lysyl Hydroxylase MT Receptor NADPH oxidase others P2 Receptor PAFR PCSK9 RAAS RANK Ligand Reverse Transcriptase S6 Kinase Thioredoxin Tie-2 SARM Vasopressin Receptor VDA Vitamin D Related CDA TrxR PDE IDO

PI3K/Akt/mTOR

Akt MELK mTOR GSK-3 PDK-1 PI3K PIKfyve PTEN RSK AMPK PI4K PIN1

Protein Tyrosine Kinase/PTK

ACK ALK Axl Bcr-Abl BTK cAMP(PKA) c-Kit c-Met CSF-1R DYRK EGFR Ephrin receptor FAK FGFR FLT3 HER2 IGF-1R MET Insulin Receptor PDGFR PKA ROS Src Syk Trk receptor VEGFR

Stem Cell /Wnt

c-Jun Hedgehog/Smoothened Hippo pathway Met stemness kinase OCT Wnt/β-catenin Adenylate Cyclase YAP PKA TGF-beta/Smad Gli Wnt sFRP-1 Smo Organoid Hedgehog Porcupine PKG Hippo (MST) Oct3/4 β-catenin BMI1

TGF-beta/Smad

TGF-beta/Smad TGF-β Receptor PKC PKA

Cell Cycle/DNA Damage

Antifolate APC ATM/ATR Aurora Kinase BMI-1 Casein Kinase CDK CD38 Chk DHFR DNA alkylator DNA-PK DNA/RNA Synthesis DUB Dynamin HDAC HSP Kinesin Microtubule Associated p97 MTH1 PAK PERK PLK PPAR PARP ROCK Sirtuin RAD51 Telomerase TOPK Topoisomerase Wee1 Deubiquitinase NEKs OGG Polo-like Kinase (PLK) Microtubule/Tubulin Mps1 MASTL Haspin Kinase SRPK DNA Alkylator/Crosslinker ATF6 Checkpoint Kinase (Chk) G-quadruplex Nucleoside Antimetabolite/Anal Eukaryotic Initiation Factor ( CRISPR/Cas9 IRE1 MARK Epoxide Hydrolase

Protein Tyrosine Kinase/RTK

TAM Receptor ROS Kinase JAK Src VEGFR c-Met Syk ALK FAK IGF-1R FLT3 c-Met/HGFR PDGFR Bcr-Abl c-Fms Ephrin receptor FLT3 BTK Itk Anaplastic lymphoma kinase (AL Ack1 RET c-Kit FGFR EGFR Insulin Receptor Discoidin Domain Receptor BRK BMX Kinase SHP2 DYRK Tie Pyk2 GDNF Receptor

Epigenetics Histone

Methyltransferase

Anti-infection

HSV Flavivirus others Fungal Parasite HBV Bacterial Dengue virus HIV Filovirus Antibiotic VSV SARS-CoV Beta-lactamase HCV CMV Reverse Transcriptase Influenza Virus Arenavirus HIV Protease

MAPK/ERK Pathway

ERK p38 MAPK MEK MAP3K Ribosomal S6 Kinase (RSK) Raf JNK MAP4K KLF Mixed Lineage Kinase MNK

Vitamin D Related/Nuclear Rece

Estrogen Receptor/ERR Progesterone Receptor VD/VDR Androgen Receptor Thyroid Hormone Receptor Nuclear Hormone Receptor 4A/NR Constitutive Androstane Recept

Cytoskeleton

MARCKS Integrin Gap Junction Protein Myosin Polo-like Kinase (PLK) Annexin A Dynamin Arp2/3 Complex

Antibodydrug Conjugate/ADC Rel

ADC Cytotoxin Antibody-Drug Conjugates (ADCs Drug-Linker Conjugates for ADC

PROTAC

Molecular Glues PROTACs Ligands for Target Protein for PROTAC Linkers Ligands for E3 Ligase E3 Ligase Ligand-Linker Conjug PROTAC-Linker Conjugates for P Target Protein Ligand-Linker C SNIPERs LYTACs AUTOTACs

通用试剂类

氨基酸

氨基酸犬尿氨酸系列

胆酸系列

蛋白及衍生物

合成多肽

核酸及其衍生物

抗生素及抗病毒

抗生素及抗病毒抗真菌抗生素抗细菌抗生素抗肿瘤抗生素抗病毒化合物

酶辅酶

染色剂指示剂

生物缓冲剂

碳水化合物

碳水化合物多糖寡糖去垢剂

通用试剂

微生物检测

微生物培养基

维生素

显色及标记试剂

植物生长激素

其他生化试剂

其他生化试剂表面活性剂无机盐

还原剂

蛋白修饰

交联剂

表面活性剂/去垢剂

非离子表活剂/去垢剂阴离子表活剂/去垢剂阳离子表活剂/去垢剂

生物耗材

吸头类

常规吸头滤芯吸头（PCR用）

PCR类

离心管类

常规离心管

细胞培养类

细胞培养板细胞培养皿细胞冻存培养瓶

液体处理类

移液管

体外诊断

核心原料

亲和素

植物研究相关

植物激素

赤霉素类(GA) 细胞分裂素(CTK) 油菜素甾醇(BR) 其他植物激素脱落酸（ABA）乙烯(ETH)

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VX11e

总货号: MB5529

货号	规格	发货时间
MB5529	5MG [￥950.00] :￥950.00	现货

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摩尔浓度计算器

分子量	500.35
化学式	C24H20Cl2FN5O2
CAS号	896720-20-0
稳定性	3 years -20°C powder
稳定性	2 years -80°C in solvent
别名	VTX-11e, Vertex-11e

DMSO	100 mg/mL (199.86 mM)
Ethanol	16 mg/mL (31.97 mM)
Water	Insoluble

激酶实验：	ERK抑制试验: 通过分光光度偶联酶试验测定化合物对ERK2的抑制。在该试验中，固定浓度的活化ERK2 (10 nM)与不同浓度化合物的DMSO (2.5%)溶液于30 °C，在pH = 7.5，包含10 mM MgCl2，2.5 mM磷酸烯醇丙酮酸盐，200 µM NADH，150 µg/mL 丙酮酸激酶，50 µg/mL乳酸脱氢酶和200 µM erktide多肽的0.1 M HEPES缓冲液中培育10分钟。反应通过加入65 µM ATP起始。监测340 nM下的吸光度减少率。IC50值根据抑制剂浓度估算。
细胞实验：	Cell lines: HT29细胞 Concentrations: ~10 μM Incubation Time: 48小时 Method: 细胞增殖通过3H-胸苷整合法测量。将细胞以10,000细胞/孔的浓度使用包含10% FBS的生长培养基RPMI 1640接种于96孔板。加入连续稀释的化合物。细胞和化合物在37°C培养箱中培育48小时。48小时后，将0.4 µCi 3H-胸苷加入每个孔，培育8小时，然后将其放回37°C培养箱。细胞使用Tomtec 96-孔细胞采集器采集，CPM使用Wallac 1205 BETAPLATE液体闪烁计数器测定。IC50是对照组的50%抑制值。
动物实验：	Animal Models: 负荷人黑色素瘤 RPDX 肿瘤的 NSG 小鼠 Formulation: 5% 乙醇，20% 丙二醇，7.4% Tween80 Dosages: 50 mg/kg BID Administration: p.o.

浓度/ 溶剂体积(DMSO)/ 质量	1 mg	5 mg	10 mg
1 mM	1.9986 mL	9.9930 mL	19.9860 mL
5 mM	0.3997 mL	1.9986 mL	3.9972 mL
10 mM	0.1999 mL	0.9993 mL	1.9986 mL
50 mM	0.0400 mL	0.1999 mL	0.3997 mL