大连美仑生物技术有限公司

Apoptosis Bcl-2 Bcl-6 Caspase Ferroptosis Mdm2 p53 RIP kinase Survivin TNF-alpha Cuproptosis IAP Metabolic Enzyme/Protease PDHK ROS Kinase Phosphatase MDM-2/p53 Kinesin PKC HSP Autophagy TNF Receptor FKBP Bcl-2 Family Pyroptosis DAPK PKD EBV Glutathione Peroxidase Transferrin Receptor c-Myc Necroptosis ASK1 Thymidylate Synthase

Autophagy

Autophagy LRRK2 Estrogen/progestogen PINK1/Parkin Dynamin Anti-infection Oxidative Phosphorylation Akt AMPK JAK Toll-like Receptor (TLR) ULK p62 Atg8/LC3 Atg7 Atg4 AUTACs

Epigenetics

DNA Methyltransferase DNMT Epigenetic Reader Domain Histone Acetyltransferase Histone demethylases Histone Methyltransferase PKC AMPK HDAC JAK MicroRNA Protein Arginine Deiminase Phosphodiesterase (PDE)

GPCR/G Protein

Adenosine Receptor Angiotensin Receptor Cannabinoid Receptor CaSR E1 Activating CRFR E2 conjugating E3 Ligase Endothelin Receptor GHSR Glucagon Receptor GLP-1R GPCR19(TGR5) GPR GPR119 GPR40(FFAR1) Imidazoline Receptor Leukotriene Receptor LPA Receptor OX Receptor Protease-activated Receptor Rac Prostaglandin Receptor Oxytocin Receptor Ras Rho S1P Receptor Sigma Receptor 5-HT Receptor Neurokinin Receptor mAChR GCGR LPL Receptor Dopamine Receptor Guanylate Cyclase Histamine Receptor CXCR CCR GPR35 P2Y Receptor Opioid Receptor GPR68 mGluR Formyl Peptide Receptor (FPR) Platelet-activating Factor Rec GnRH Receptor Protease Activated Receptor (P Vasopressin Receptor Chemerin Receptor Melatonin Receptor MCHR1 (GPR24) Orexin Receptor (OX Receptor) RGS Protein CGRP Receptor Free Fatty Acid Receptor

Immunology/Inflammation

AhR CCR COX Complement 5(C5) CXCR Histamine Receptor IL Receptor Immunology IL-5 NOD1 IL-12 PD-1 IL-17 PDL-1 reactive oxygen species (ROS) NLRP3 TLR STING Toll-like Receptor (TLR) Bcr-Abl Salt-inducible Kinase (SIK) Glucocorticoid Receptor IRAK SphK Interleukin Related PGE synthase Macrophage migration inhibitor Complement System MyD88 FLAP Thrombopoietin Receptor Arginase NO Synthase AP-1 Nuclear Factor of activated T IFNAR CD276/B7-H3 CD3 CD38 CD28 CD22 CD20 CD19 Aryl Hydrocarbon Receptor PD-1/PD-L1 NOD-like Receptor (NLR) Mesothelin Mucin NTPDase PSMA SOD DOCK CD74 CD6 CD73 CTLA-4 CX3CR1 Cyclic GMP-AMP Synthase FAP Galectin Cyclophilin P-selectin CD2 CD47 Tim3 TROP2 Glycoprotein VI Kallikrein LAG-3 VAP-1 BCL6

Integrin

JAK-STAT/IL6

JAK STAT IL-6 Pim EGFR

MAPK

ErbB2 ERK JNK MEK p38 MAPK Mixed Lineage Kinase Raf

Membrane Transporter/Ion Chann

Amino acid transporter antagon ATPase Calcium Channel CFTR Chloride channel CRM1 GABA Receptor GluR iGluR NMDAR P-gp Potassium Channel Proton Pump SGLT Sodium Channel TRPV P-glycoprotein Na+/K+ ATPase Piezo Channel Fat Mass and Obesity-associate TRP Channel BCRP nAChR CRAC Channel Apical Sodium-Dependent Bile A P2X Receptor Monocarboxylate Transporter Na+/HCO3- Cotransporter GlyT OAT NKCC Monoamine Transporter HCN Channel EAAT Ferroportin GLUT Na+/H+ Exchanger (NHE) Na+/Ca2+ Exchanger FATP ATP Synthase ASCT VDAC Urea Transporter URAT1 Aquaporin Calmodulin

Metabolic Enzyme/Protease

5-alpha Reductase Aminopeptidase ATP-citrate lyase in Carbonic Anhydrase CETP DGAT DPP-4 Factor Xa FOX FXR Glucokinase(GK) GSNOR HCV Protease HIF HMG-CoA Reductase HSP IDO Lactate Dehydrogenase Integrase MMP NAMPT P450 PAI-1 PDE PGC-1α Phospholipase Procollagen C Protein Proteases PKM Proteasome RAR/RXR Retinoid Receptor Serine Protease Thrombin Transferase Xanthine Oxidase p38-MAPK Stearoyl-CoA Desaturase (SCD) Mitochondrial Metabolism (MPC) Tyrosinase Endogenous Metabolite Phosphatase Pyruvate Kinase PDI Phosphodiesterase (PDE) HIF/HIF Prolyl-Hydroxylase Hexokinase Ser/Thr Protease Carboxylesterase Cathepsin Cytochrome P450 Drug Metabolite E1/E2/E3 Enzyme Angiotensin-converting Enzyme HMG-CoA Reductase (HMGCR) Aldose Reductase Oxidative Phosphorylation NADPH Oxidase Lipoxygenase DAGL MAGL MALT1 Adenosine Kinase COMT Glutathione S-transferase Adenosine Deaminase Acyltransferase Enolase Aldehyde Dehydrogenase (ALDH) Farnesyl Transferase Glucosidase PDHK Fatty Acid Synthase (FASN) Dipeptidyl Peptidase Methylenetetrahydrofolate Dehy Dihydroorotate Dehydrogenase Renin FAAH SGK Dopamine β-hydroxylase HIV Integrase Adiponectin Receptor ROR LXR Mineralocorticoid Receptor REV-ERB Amine N-methyltransferase

Neuronal Signaling

5-HT Receptor AChR BACE Beta Amyloid Beta-secretase CaMK CGRP Receptor Dopamine Receptor FAAH Gamma-secretase GlyT iGluR MAO mAChR Opioid Receptor Serotonin Transporter Legumain γ-secretase Notch Monoamine Oxidase Trk Receptor Cholinesterase (ChE) Imidazoline Receptor Amyloid-β Dopamine Transporter Sigma Receptor Glucosylceramide Synthase (GCS

NF-κB

NF-κB Nrf2 IκB/IKK Reactive Oxygen Species Keap1-Nrf2 IKK RANKL/RANK

Others

Androgen Receptor Aromatase CD20 CD52 c-Myc CPT1 c-RET CTLA-4 Decarboxylase Dehydrogenase FPR GLUT Glutaminase HIV ProteaseAnti-infection HSP90 Hydroxylase IAP IgE IRE1 LDL Lipase Lipoxygenase Lysyl Hydroxylase MT Receptor NADPH oxidase others P2 Receptor PAFR PCSK9 RAAS RANK Ligand Reverse Transcriptase S6 Kinase Thioredoxin Tie-2 SARM Vasopressin Receptor VDA Vitamin D Related CDA TrxR PDE IDO

PI3K/Akt/mTOR

Akt MELK mTOR GSK-3 PDK-1 PI3K PIKfyve PTEN RSK AMPK PI4K PIN1

Protein Tyrosine Kinase/PTK

ACK ALK Axl Bcr-Abl BTK cAMP(PKA) c-Kit c-Met CSF-1R DYRK EGFR Ephrin receptor FAK FGFR FLT3 HER2 IGF-1R MET Insulin Receptor PDGFR PKA ROS Src Syk Trk receptor VEGFR

Stem Cell /Wnt

c-Jun Hedgehog/Smoothened Hippo pathway Met stemness kinase OCT Wnt/β-catenin Adenylate Cyclase YAP PKA TGF-beta/Smad Gli Wnt sFRP-1 Smo Organoid Hedgehog Porcupine PKG Hippo (MST) Oct3/4 β-catenin BMI1

TGF-beta/Smad

TGF-beta/Smad TGF-β Receptor PKC PKA

Cell Cycle/DNA Damage

Antifolate APC ATM/ATR Aurora Kinase BMI-1 Casein Kinase CDK CD38 Chk DHFR DNA alkylator DNA-PK DNA/RNA Synthesis DUB Dynamin HDAC HSP Kinesin Microtubule Associated p97 MTH1 PAK PERK PLK PPAR PARP ROCK Sirtuin RAD51 Telomerase TOPK Topoisomerase Wee1 Deubiquitinase NEKs OGG Polo-like Kinase (PLK) Microtubule/Tubulin Mps1 MASTL Haspin Kinase SRPK DNA Alkylator/Crosslinker ATF6 Checkpoint Kinase (Chk) G-quadruplex Nucleoside Antimetabolite/Anal Eukaryotic Initiation Factor ( CRISPR/Cas9 IRE1 MARK Epoxide Hydrolase

Protein Tyrosine Kinase/RTK

TAM Receptor ROS Kinase JAK Src VEGFR c-Met Syk ALK FAK IGF-1R FLT3 c-Met/HGFR PDGFR Bcr-Abl c-Fms Ephrin receptor FLT3 BTK Itk Anaplastic lymphoma kinase (AL Ack1 RET c-Kit FGFR EGFR Insulin Receptor Discoidin Domain Receptor BRK BMX Kinase SHP2 DYRK Tie Pyk2 GDNF Receptor

Epigenetics Histone

Methyltransferase

Anti-infection

HSV Flavivirus others Fungal Parasite HBV Bacterial Dengue virus HIV Filovirus Antibiotic VSV SARS-CoV Beta-lactamase HCV CMV Reverse Transcriptase Influenza Virus Arenavirus HIV Protease

MAPK/ERK Pathway

ERK p38 MAPK MEK MAP3K Ribosomal S6 Kinase (RSK) Raf JNK MAP4K KLF Mixed Lineage Kinase MNK

Vitamin D Related/Nuclear Rece

Estrogen Receptor/ERR Progesterone Receptor VD/VDR Androgen Receptor Thyroid Hormone Receptor Nuclear Hormone Receptor 4A/NR Constitutive Androstane Recept

Cytoskeleton

MARCKS Integrin Gap Junction Protein Myosin Polo-like Kinase (PLK) Annexin A Dynamin Arp2/3 Complex

Antibodydrug Conjugate/ADC Rel

ADC Cytotoxin Antibody-Drug Conjugates (ADCs Drug-Linker Conjugates for ADC

PROTAC

Molecular Glues PROTACs Ligands for Target Protein for PROTAC Linkers Ligands for E3 Ligase E3 Ligase Ligand-Linker Conjug PROTAC-Linker Conjugates for P Target Protein Ligand-Linker C SNIPERs LYTACs AUTOTACs

通用试剂类

氨基酸

氨基酸犬尿氨酸系列

胆酸系列

蛋白及衍生物

合成多肽

核酸及其衍生物

抗生素及抗病毒

抗生素及抗病毒抗真菌抗生素抗细菌抗生素抗肿瘤抗生素抗病毒化合物

酶辅酶

染色剂指示剂

生物缓冲剂

碳水化合物

碳水化合物多糖寡糖去垢剂

通用试剂

微生物检测

微生物培养基

维生素

显色及标记试剂

植物生长激素

其他生化试剂

其他生化试剂表面活性剂无机盐

还原剂

蛋白修饰

交联剂

表面活性剂/去垢剂

非离子表活剂/去垢剂阴离子表活剂/去垢剂阳离子表活剂/去垢剂

生物耗材

吸头类

常规吸头滤芯吸头（PCR用）

PCR类

离心管类

常规离心管

细胞培养类

细胞培养板细胞培养皿细胞冻存培养瓶

液体处理类

移液管

体外诊断

核心原料

亲和素

植物研究相关

植物激素

赤霉素类(GA) 细胞分裂素(CTK) 油菜素甾醇(BR) 其他植物激素脱落酸（ABA）乙烯(ETH)

您所在的位置: 首页 > 信号通路研究 > Immunology/Inflammation > PD-1

非常规包装或大包装请单独咨询

Nivolumab

总货号: MB2767

货号	规格	发货时间
MB2767-1	1MG/只 [￥900.00] :￥900.00	现货
MB2767-2	5MG/只 [￥3450.00] :￥3450.00	现货

数量

立即购买

加入购物车

产品问答

产品简介

产品参数

质检证书

下载说明书

产品图谱

摩尔浓度计算器

相关论文

分子量：143.6 KDa

简介：Nivolumab(anti-PD-1)是一种人源化抗PD-1的IgG4单克隆抗体类抑制剂，具有免疫检查点抑制活性和抗肿瘤活性，可用于晚期（转移性）非小细胞肺癌的研究。

产品形式：蛋白原液，装于冻存管中，单抗质量为标示数量1mg或者5mg，体积根据不同批次浓度不同略有不同，具体批次浓度请详见COA。

别名：纳武单抗; 尼沃单抗; 纳武利尤单抗; BMS-936558; ONO-4538; MDX-1106

物理性质和指标：

外观：……………….......Liquid

克隆类型：………….......重组抗体

表达系统：………….......HEK293 supernatant

亚型：……………….......Human IgG4SP

储存缓冲液：………..…..Acetic acid buffer, pH5.4 with protectants

浓度：……………….......见COA

纯度：……………..........Non-reduced SDS-PAGE：≥95%；SEC-HPLC：≥95%

内毒素：……………...…Less than 10 EU/mg by LAL method

Biological Activity：……Corresponds to reference standard

产品用途：科研试剂，广泛应用于分子生物学、药理学等科研方面，严禁用于人体。Nivolumab的核心作用是阻断PD-1/PD-L1通路，解除肿瘤细胞对免疫系统的抑制，恢复机体抗肿瘤免疫活性，科研主要用于多种恶性肿瘤的研究，如：黑色素瘤、非小细胞肺癌、肾细胞癌、尿路上皮癌、霍奇金淋巴瘤、头颈部鳞状细胞癌等。

储存条件：-70±15℃避光保存。首次使用后建议分装，避免反复冻融。

运输条件：干冰运输

生物活性：

Target

PDCD1/PD-1/CD279

体外研究

(1) Nivolumab与PD-1以高亲和力结合（Kd=2.6 nM），阻止其与B7-H1和B7-DC的相互作用。

(2) 应用FACS来检测CHO细胞中PD-1的配体结合情况的实验中，Nivolumab抑制PD-1与PD-L1或PD-L2结合的效力相似（IC₅₀值相似，分别为1.04 nM和0.97 nM）。Nivolumab与PD-1特异性结合，而不与其他免疫球蛋白超家族蛋白如CD28、CTLA-4、ICOS和BTLA作用。

(3) 在存在T细胞受体刺激的情况下，低浓度(~1.5 ng/mL)的Nivolumab可增强T细胞反应活性；在缺少抗原或T细胞受体刺激的情况下，Nivolumab没有刺激效果。

(4) 在未受刺激的全血中，Nivolumab不会引起炎性因子（包括IFNγ, TNFα, IL-2, IL-4, IL-6或IL-10）的显著释放。Nivolumab也不会造成非特异性淋巴细胞的激活。

体内研究

在猴子中，血清中Nivolumab具有相对较慢的清除率和有限的血管外分布。给药浓度为1 mg/kg时，在雄性和雌性中Nivolumab的表观终末消除半衰期类似，分别为124和139小时；给药浓度为10 mg/kg时，Nivolumab在雄性体内的平均半衰期为261小时。尽管在猴子中Nivolumab看似没有毒性作用，但在人类临床试验中观察到了其毒性作用。在临床I期试验中，Nivolumab安全性良好。副作用与ipilimumab类似，尽管发生率较低，严重性较低，副作用包括胃肠道的、内分泌和皮肤毒性以及肺部炎症。肺炎只在具有PD-1缺陷的MRL遗传背景的小鼠中出现，而没有在其他遗传背景的PD-1缺陷型小鼠中观察到。

使用方法：请详见说明书

【注意】

●本产品为无菌包装，使用时请注意无菌操作，防止污染。

●为了您的安全和健康，请穿实验服并戴一次性手套操作。

●部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，以上数据仅供参考交流研究之用。

参考文献：

[1]. Wang C, et al. In vitro characterization of the anti-PD-1 antibody nivolumab, BMS-936558, and in vivo toxicology in non-human primates. Cancer Immunol Res. 2014 Sep;2(9):846-56.

[2]. The Luc Ma, Stephanie Daignault, Jessica Waninger, et al., The impact of BRAF mutation status on clinical outcomes with single-agent PD-1 inhibitor versus combination ipilimumab/nivolumab, 38(15_suppl) (2020) 10024-10024.

[3]. Wei Chi, Lianyong Zhang, Xue Wang, et al., Effects of Nivolumab and Ipilimumab on the suppression of cisplatin resistant small cell lung cancer cells, (4) (2022) 40.

生物活性数据展示：

Immoblized PD-1 at 1μg/mL binding to Nivolumab (Meilunbio, Cat:MB2767), and tested by anti-Human IgG Fc Antibody [HRP] at 1:10000. The binding EC₅₀ is 0.269 nM and converts to 38.557 ng/mL.

相关产品推荐：

产品编号	产品名称	Target
MB2773	Infliximab	TNF-α
MB2806	Aflibercept	VEGFR
MB2767	Nivolumab	PD-1
MB2749	Rituximab	CD20
MB2791	Bevacizumab	VEGF
MB2786	Alemtuzumab	CD52
MB2787	Alirocumab	PCSK9
MB2789	Avelumab	PDL-1
MB2790	Atezolizumab	PDL-1
MB2792	Cetuximab	EGFR
MB2803	Daratumumab	CD38
MB2794	Denosumab	RANK Ligand
MB2023	Eculizumab	补体蛋白(C5)
MB2795	Etanercept	TNF
MB2797	Evolocumab	PCSK9
MB2769	Ipilimumab	CTLA-4
MB2772	Matuzumab	EGFR
MB2766	Mepolizumab	IL-5
MB2784	Adalimumab	TNF-α
MB2774	Obinutuzumab	CD20
MB2776	Ofatumumab	CD20
MB2777	Omalizumab	IgE
MB2781	Panitumumab	EGFR
MB2761	Pembrolizumab	PD-1
MB2762	Pertuzumab	HER2
MB2763	Ranibizumab	VEGFR
MB2938	Ramucirumab	VEGFR
MB2757	Secukinumab	IL-17
MB2751	Tocilizumab/Atlizumab	IL-6 receptor
MB2753	Trastuzumab	ErbB2
MB2758	Ustekinumab	IL-12

我司所售出产品仅供于科研研究用途（非临床科研研究），每次销售产品行为都适用于我司网上所列明的通用销售条款。

CAS号：946414-94-4

别名：纳武单抗; BMS-936558; ONO-4538; MDX-1106

质量标准：>95%,蛋白原液

摩尔浓度计算器（本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系）

摩尔浓度计算公式：质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

质量（g）

浓度 (mol/L)

体积 (L)

分子量 (g/mol)